Complete mitochondrial genome of Onchocerca lupi (Nematoda, Onchocercidae)

Onchocerca lupi, Rodonaja 1967, is an emerging, zoonotic filarial nematode parasite that causes ocular disease in dogs, cats, wild canids, and humans. It is the causative agent of ocular onchocercosis in canines with increasing incidence in both North America and the Old World during the early twenty-first century. We report the complete mitochondrial genome of an O. lupi isolate from a dog from Arizona, southwestern USA, and its genetic differentiation from related Onchocerca species. The whole mitochondrial genome was obtained from whole genome sequencing of genomic DNA isolated from an adult worm. This mitogenome is 13,766 bp in size and contains 36 genes and a control region. This mitogenome provides a valuable resource for future studies involving epidemiological surveillance, population genetics, phylogeography, and comparative mitogenomics of this emerging pathogen and other parasitic nematodes

Primer sequences for quantitative and conventional PCR for both host and parasite used in this study.

Primer sequences for quantitative and conventional PCR for both host and parasite used in this study.</p

qPCR predicted host-to-parasite DNA ratios, WGS read alignment percentages, and from three adult <i>O</i>. <i>lupi</i> nematodes.

*Reported ratio is not based on total read numbers.</p

Accession information for read data used for parasite locus primer design.

(DOCX)</p

Comparison of <i>Onchocerca lupi</i> percentage from three biological canine samples.

Samples were screened using LupiQuant which estimated O. lupi percentages within each sample. Whole genome sequences of O. lupi samples were aligned to O. lupi reference genome (BioProject PRJNA802584) to compare LupiQuant ratios. Error bars represent the range of O. lupi percentages based on host and parasite technical replicates.</p

Accession information for polycystin-1 precursor (<i>pkd1</i>) gene sequences included for host locus primer design.

(DOCX)</p